Caspase-3, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) from Abcam

Product Specs
ItemCaspase-3, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab219915
Quantity100 test
Sample TypeAdherent cells, Suspension cells
ApplicationsCellular Assay
Gene NameCASP8
TypeSemi-quantitative
NCBI Gene AliasesALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5
TargetCaspase-3 + Caspase-8 + Caspase-9

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) A Dual Concentration-Tailored Cytokine-Chemo Nanosystem to Alleviate Multidrug Resistance and Redirect Balance of Cancer Proliferation and ApoptosisInternational Journal of NanomedicineJuly 28, 2023Yu Hsia, Maharajan Sivasubramanian, Chia-Hui Chu, Yao-Chen Chuang, Yiu-Kay Lai, Leu-Wei LoActivity Assay Caspase-3, caspase-8 and caspase-9 Multiplex Activity Assay Kit (Fluorometric) (Abcam, ab219915) provide a tool to quantify caspase-3, −8, −9 activity in cells that are undergoing apoptosis. This

(2) Two new Cd(II)/Zn(II) coordination polymers: luminescence properties and synergistic treatment activity with ultrasound therapy on uterine fibroidsDesigned Monomers and PolymersDecember 31, 2022Hong-Mei Liu, Xiao-Na Shangspectrophotometer at room temperature. Caspase-3 Activity Assay Kit (ab252897) and Caspase-8 Activity Assay Kit (ab219915) were purchased from the Abcam. Synthesis of [Cd(5-meo-ip)(bmip)]n (1) and [Zn(5-pro-ip)(bip)]n·2n(H2O)(2) A

(3) Cytoplasmic sequestration of p53 by lncRNA-CIRPILalleviates myocardial ischemia/reperfusion injuryCommunications BiologyDecember 1, 2022Yuan Jiang et al.reader. To detect the activity of caspase-8, caspase-9, another detection kit was used (Abcam, USA, #ab219915) according to the manufacturer’s protocol. Briefly, caspase assay solution was added to cells and incubated

(4) In Vitro Cell Death Mechanisms Induced by Dicoma anomala Root Extract in Combination with ZnPcS(4) Mediated-Photodynamic Therapy in A549 Lung Cancer CellsCellsOctober 19, 2022Chota A, George BP, Abrahamse H.the activities of caspase 8 and 9 were evaluated using a fluorometric multiplex activity assay kit (ab219915) (Abcam). The assay substrates were prepared by following the manufacturer’s instructions. 24 h post-treatment,

(5) ISG15 deficiency features a complex cellular phenotype that responds to treatment with itaconate and derivativesClinical and Translational MedicineJuly 17, 2022Syed Fakhar‐ul‐Hassnain Waqas et al.calculations of OCR. 2.13 Fluorometric assay A caspase multiplex fluorometric assay kit (Abcam; cat# ab219915) was used to measure caspase activity to detect cells undergoing apoptosis. Briefly, cells were seeded

(6) Fisetin Deters Cell Proliferation, Induces Apoptosis, Alleviates Oxidative Stress and Inflammation in Human Cancer Cells, HeLaInternational Journal of Molecular SciencesFebruary 1, 2022Nazia Afroze, Sreepoorna Pramodh, Jasmin Shafarin, Khuloud Bajbouj, Mawieh Hamad, Madhumitha Kedhari Sundaram, Shafiul Haque, Arif Hussainand 9) assay was performed. The caspase multiplex assay kit (fluorometric) was obtained from Abcam (ab219915; Cambridge, UK), and the assay was completed according to the kit’s protocol. Briefly, ~8 × 103 cells/per

(7) Distinct mechanism of cervical cancer cell death caused by the investigational new drug SHetA2Frontiers in oncologyJanuary 1, 2022Rai R, Chandra V, Kennedy AL, Zuna RE, Benbrook DM.activity was measured using Caspase 3, Caspase 8, and Caspase 9 Multiplex Activity Assay Kit (Fluorometric#ab219915, Abcam, Cambridge, MA, USA). Briefly, 1.5 X 104 cells were seeded in 96-well black plate for overnight

(8) Response of MCF-7 Breast Cancer Cells Overexpressed with P-Glycoprotein to Apoptotic Induction after Photodynamic TherapyMoleculesDecember 6, 2021Eric Chekwube Aniogo, Blassan P. George, Heidi Abrahamsereader, Pontyclun, UK). 2.6. Fluorometric Measurement of Caspase 8 and 9 This multiplex assay kit (ab219915) from Abcam provides a simple and convenient tool for quantifying caspase 8 and caspase 9 activity in

(9) Circ_0001821 knockdown suppresses growth, metastasis, and TAX resistance of non‐small‐cell lung cancer cells by regulating the miR‐526b‐5p/GRK5 axisPharmacology Research & PerspectivesAugust 1, 2021Ying Liu, Changchao Li, Huiqun Liu, Jing WangV‐FITC + and PI − or +. The activity of caspase‐3 and caspase‐9 was measured utilizing a commercial kit (ab219915; Abcam). According to the protocol booklet supplied by the manufacturer, treated A549 and H3122 cells

(10) Cytotoxic Effects and Intracellular Localization of Bin Toxin from Lysinibacillus sphaericus in Human Liver Cancer Cell LineToxins (Basel)April 19, 2021Simab Kanwal, Shalini Abeysinghe, Monrudee Srisaisup, Panadda Boonsermto protein toxins was done using Caspase 3, Caspase 8 and Caspase 9 Multiplex Activity Assay Kit (Abcam, ab219915) according to the manufacturer’s protocol. Briefly, Hs68 and HepG2 cells were applied to each of the

(11) Alternol Sensitizes Renal Carcinoma Cells to TRAIL-Induced ApoptosisFrontiers in PharmacologyMarch 25, 2021Yu Ren, Xue Wang, Shuaishuai Huang, Yangkai Xu, Guobin Weng, Rui Yuactivity were determined using a caspase-3 and -8 multiplex activity assay kit (Fluorometric) (cat no ab219915; Abcam; Cambridge, United States). Briefly, after different treatments, cells were washed with PBS three

(12) Hydrogen sulfide inhibits endoplasmic reticulum stress through the GRP78/mTOR pathway in rat chondrocytes subjected to oxidative stressInternational Journal of Molecular MedicineFebruary 2, 2021Jianjun Wu, Fan Yang, Xin Zhang, Guanghua Chen, Jilong Zou, Li Yin, Dawei Yangactivities of caspase-3, caspase-8 and caspase-9 were determined using a Multiplex Activity Assay kit (ab219915, Abcam) according to the manufacturer's instructions with some modifications. The protocol in the following

(13) Regulation of Anti-Apoptotic SOD2 and BIRC3 in Periodontal Cells and TissuesInternational Journal of Molecular SciencesJanuary 8, 2021Birgit Rath-Deschner et al.Activity Caspase activity was quantified using the fluorometric kit Caspase 3 Multiplex Activity Assay (ab219915, Abcam) according to the manufacturer’s protocol. Cells from the PDL cell line PDL26 were grown in a

(14) SARS-CoV-2 Accessory Protein ORF7b Mediates Tumor Necrosis Factor-α-Induced Apoptosis in CellsFrontiers in microbiologyJanuary 1, 2021Yang R, Zhao Q, Rao J, Zeng F, Yuan S, Ji M, Sun X, Li J, Yang J, Cui J, et al.Multiplex Activity Assay Kit for caspase 3, caspase 8, and caspase 9 according to the manufacturer’s (ab219915, Abcam) instructions. ELISA kits for human IL-6 (KE00007; ProteinTech), TNF-α (KE00068; ProteinTech),

(15) Determining the effect of ellagic acid on the proliferation and migration of pancreatic cancer cell linesTranslational Cancer ResearchJanuary 1, 2021Ji Yoon Kim, Yun Ju Choi, Ha-Jeong KimCaspase-3, caspase-8, and caspase-9 activities were determined using caspase multiplex kit from Abcam (ab219915) following the manufacture’s protocol. SpectraMax (Molecular Devices) was used to detect fluorescence.

(16) Prevention of dsRNA-induced interferon signaling by AGO1x is linked to breast cancer cell proliferationThe EMBO journalSeptember 15, 2020Ghosh S, Guimaraes JC, Lanzafame M, Schmidt A, Syed AP, Dimitriades B, Börsch A, Ghosh S, Mittal N, Montavon T, et al.(caspase 8 and caspase 9) and the executioner caspase 3, was performed with kits obtained from Abcam (ab219915). The kit uses DEVD‐ProRed™, IETD‐R110, and LEHD‐AMC as fluorogenic indicators for caspase 3, caspase

(17) Zn2+-dependent suppression of vascular smooth muscle intimal hyperplasia from biodegradable zinc implantsMaterials Science and Engineering: CJune 1, 2020Roger J. Guillory, Timothy M. Kolesar, Alexander A. Oliver, Jeffrey A. Stuart, Martin L. Bocks, Jaroslaw W. Drelich, Jeremy Goldmandetected from the medial compartment of the arterial rings using a plate based fluorescent assay (Abcam, ab219915). Protein determination was carried out using a Bradford assay kit (Abcam, ab102535). There was a minimum

(18) H2S attenuates endoplasmic reticulum stress in hypoxia-induced pulmonary artery hypertensionBioscience ReportsJuly 31, 2019Jianjun Wu, Weili Pan, Chao Wang, Hui Dong, Lei Xing, Jingbo Hou, Shaohong Fang, Hulun Li, Fan Yang, Bo Yuapoptotic response. Caspase 3, Caspase 8 and Caspase 9 Multiplex Activity Assay Kit (Fluorometric) (ab219915) are purchased by Abcam. It a fluorescent assay that detects the activity of caspase-3/-8/-9 in cell

(19) The role of chamaejasmine in cellular apoptosis and autophagy in MG-63 cellsBioscience ReportsJanuary 31, 2019Dawei Yang, Hao Zhang, Jianjun Wu, Ruishuang Ma, Zongyu Li, Kunzheng Wang, Fan Yangapoptotic response. Caspase 3, Caspase 8, and Caspase 9 Multiplex Activity Assay Kit (Fluorometric) (ab219915) were purchased by Abcam. It a fluorescent assay that detects the activity of caspase 3/ 8/ 9 in cell

(20) Polydatin exerts anti-tumor effects against renal cell carcinoma cells via induction of caspase-dependent apoptosis and inhibition of the PI3K/Akt pathwayOncoTargets and TherapyDecember 12, 2018Yi-Li Jin, Li-Min Xin, Chang-Chun Zhou, Yu Renof caspase-3 and caspase-9 were determined by caspase-3 and caspase-9 assay kits, respectively (No. ab219915; Abcam, Cambridge, MA, USA) according to the manufacturer’s instructions. After the treatments, 100

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Caspase-3, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) from Abcam

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Caspase-3, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) from Abcam

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